FastQCFastQC Report
Sat 1 Sep 2018
HH2GFBGX7_n01_7_tga1_col_gr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2GFBGX7_n01_7_tga1_col_gr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19736487
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT959960.4863884844349453TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT135800.057.66445549
TATGCCG138000.057.1258448
TGCCGTC136550.057.0138850
CGTATGC139000.056.9653746
GTATGCC139500.056.81312647
GTCACGT151250.054.33890529
TCACGTG153750.053.39140730
GGCCTTA151800.053.35667436
CACGTGG161850.050.68164431
TCTCGTA159700.046.59560843
GCCGTCT170200.045.225551
AGTCACG185050.044.67525528
ATCTCGT167800.044.49063542
TCGTATG176600.044.4002345
TATCTCG168650.044.36841641
GTGGCCT187650.043.71940634
CTCGTAT174550.043.2756244
CGTGGCC191300.042.62758633
GCCTTAT191500.041.71195237
TTATCTC184700.040.52795440
Produced by FastQC (version 0.11.2)