FastQCFastQC Report
Sat 1 Sep 2018
HH2GFBGX7_n01_6_nlp7_nlp7_h3k27ac.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2GFBGX7_n01_6_nlp7_nlp7_h3k27ac.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19357483
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT247570.12789369361708855TruSeq Adapter, Index 19 (97% over 40bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG49800.041.98377648
CGTATGC52300.040.44334446
GTATGCC55250.037.90548747
ATGCCGT61300.034.33606749
GCCGTCT61450.033.96579751
TCGTATG74300.028.42091645
CTCGTAT70900.027.80783844
TGCCGTC76950.027.53474250
CCGTCTT95200.022.14472252
CTGCTTG99900.021.48714659
TCTCGTA89650.021.4834543
GATCTCG102250.018.18422341
ATCTCGT106100.017.7231342
CGATCTC107550.017.22157540
ACGATCT111250.016.74240739
CTTCTGC136400.015.89148656
TGCTTGA144950.015.05055460
AACGATC125950.015.03654638
TGGGGGG24350.014.9453851
GCTTGAA158350.013.77672161
Produced by FastQC (version 0.11.2)