FastQCFastQC Report
Sat 1 Sep 2018
HH2GFBGX7_n01_5_nlp7_nlp7_gr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2GFBGX7_n01_5_nlp7_nlp7_gr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15686944
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT1033220.6586496388334145TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG145500.058.9199248
CGTCCGC146950.058.65969533
CGTATGC147500.058.4750946
GTATGCC149650.057.426447
TCCGCAC150100.057.31957235
GTCCGCA151000.056.7658734
CCGCACA149150.056.2538336
ACGTCCG153400.056.16719432
GCCGTCT149700.055.9546951
CGCACAT150650.054.55837
GTCACGT158550.054.50927729
CACGTCC161250.053.38655531
TCACGTC161950.053.3461930
ATGCCGT160750.053.3081949
TCGTATG165150.051.8223345
AGTCACG167550.051.6403228
CTCGTAT159400.051.07660744
TCTCGTA164400.048.58395843
CCGTCTT173950.048.2946252
GCACATC164600.047.4892538
Produced by FastQC (version 0.11.2)