FastQCFastQC Report
Sat 1 Sep 2018
HH2GFBGX7_n01_4_mn_nlp7_h3k27ac.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2GFBGX7_n01_4_mn_nlp7_h3k27ac.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18097732
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT1135790.6275869263618226TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG140150.058.3970148
GTATGCC145850.057.53066647
CGTATGC144200.057.48227746
ATGCCGT142400.055.11320549
TCCAGTC237550.053.09903725
TGCCGTC142550.053.01598450
CCAGTCA239300.052.59523826
CAGTCAC241600.052.1977627
GCCGTCT131900.052.0638451
CTCGTAT154600.051.87056444
ACGTCTG246900.051.62656415
ACTCCAG248150.050.97040623
CTCCAGT248750.050.74902724
TCGTATG159650.050.1206945
CGTCTGA255950.049.77283516
GCACACG258050.049.32984511
GTCACAT259600.048.76190629
CACGTCT264750.048.23841514
ACACGTC266350.047.92303513
ATGTCAG263400.047.7173234
Produced by FastQC (version 0.11.2)