FastQCFastQC Report
Sat 1 Sep 2018
HH2GFBGX7_n01_3_pn_nlp7_h3k27ac.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2GFBGX7_n01_3_pn_nlp7_h3k27ac.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18621892
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC440020.23629177958931347TruSeq Adapter, Index 11 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG68800.048.76007546
CGTATGC71000.047.24805544
GTATGCC73300.045.86216445
ATGCCGT76150.043.8714647
TGCCGTC80550.041.2142748
GCCGTCT80050.041.12150249
CGGCTAC85100.040.13905733
CACGGCT84950.039.99899731
ACGGCTA90050.038.16305532
GTCACGG88950.037.99764629
CTCGTAT85000.037.8987342
TCGTATG90400.037.1849143
AGTCACG93850.036.19792628
GCTACAT103100.031.64121435
CAGTCAC110200.030.92061227
TCTCGTA103700.030.59107241
TCACGGC113900.029.98379730
CCAGTCA114800.029.74098626
CTACATC106300.029.43863136
TCCAGTC117200.029.33986725
Produced by FastQC (version 0.11.2)