FastQCFastQC Report
Sat 1 Sep 2018
HH2GFBGX7_n01_2_mn_col_h3k27ac.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2GFBGX7_n01_2_mn_col_h3k27ac.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19933199
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC1261590.6329089475301982TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG173400.057.3768146
CGTATGC175750.056.82736644
GTATGCC179150.055.75012645
ATGCCGT180200.055.21447
GTCACGC185150.054.82277729
TGCCGTC186500.053.27361748
GCCGTCT186350.052.90251549
CACGCCA192100.052.73958631
CTCGTAT188000.052.33797542
TCGTATG192000.052.19687343
TCACGCC199700.050.7979830
CGCCAAT201300.050.6308733
AGTCACG201900.050.47937428
ACGCCAA203550.049.91357432
TCTCGTA208450.047.25220541
GCCAATA216150.046.89580534
CCAGTCA222500.045.9395826
TATCTCG214800.045.88285439
CAGTCAC223900.045.76490427
TCCAGTC225450.045.66246825
Produced by FastQC (version 0.11.2)