FastQCFastQC Report
Sat 1 Sep 2018
HH2GFBGX7_n01_24_input_5_tga1_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2GFBGX7_n01_24_input_5_tga1_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16861655
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGCA75750.019.68397334
TATGCCG22000.017.986646
GTATGCC21900.017.90867245
ACAGCAT86850.016.84662435
ACTACAG93500.016.05719632
CGTATGC20950.016.04622344
CACTACA104100.014.52254931
CATCGCG31350.014.51794139
ATGCCGT24800.014.40331547
TCACTAC120750.012.57690430
CTACAGC123550.012.43581133
GCATCGC39400.012.26127638
CATCGGG22800.011.97730239
CATCTCG46200.011.89753339
GTCACTA128550.011.86719929
ATCGCGT27700.011.75525640
TGCCGTC28700.010.98185948
AGTCACT146500.010.38869428
GCATCGG29050.010.12245838
GCATCTC54700.010.11166338