FastQCFastQC Report
Sat 1 Sep 2018
HH2GFBGX7_n01_23_input_5_nlp7_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2GFBGX7_n01_23_input_5_nlp7_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18890716
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG40950.030.7853246
CGTATGC40400.030.77054844
GTATGCC41050.030.53971545
GCCGTCT48150.026.03914649
ATGCCGT57550.022.02890647
CTCGTAT58100.020.5513942
TCGTATG64050.019.79066743
CTAATCG65500.019.1290833
TCTCGTA64700.017.75106441
CCGTCTT75300.017.20846650
TAATCGA83350.015.032968534
CGATCTC74400.014.67996138
ATCTCGT79050.014.4836440
ACTAATC98950.013.12137832
TGCCGTC100650.013.08316848
TGCTTGA107300.012.0419458
TGGGGGG22200.011.8221761
AGTCACT114750.011.25029528
GATCTCG101650.011.22822239
GCTTGAA117750.011.21132959
Produced by FastQC (version 0.11.2)