FastQCFastQC Report
Sat 1 Sep 2018
HH2GFBGX7_n01_22_biotin_tga1_180_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2GFBGX7_n01_22_biotin_tga1_180_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13462812
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACAATCTCGTATGC1777081.319991692671635TruSeq Adapter, Index 2 (97% over 36bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG509720.37861332387319974No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG378470.2811225470577767No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACAATCTCGTATG180250.13388733349318108TruSeq Adapter, Index 2 (97% over 36bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC307050.047.4312144
GTATGCC307200.047.29546445
TATGCCG308200.047.11945746
CTCGTAT288900.046.58931442
GCCGTCT307050.046.3963349
CTGCTTG302800.046.29028357
TCGTATG314200.046.08197843
TGCTTGA307250.045.96276558
GCTTGAA314650.045.32711859
ATGCCGT321900.045.13699347
CCGTCTT315600.045.10476750
TCTCGTA298600.044.7934341
TCTGCTT322500.043.5391256
TGCCGTC334000.043.21902548
CTTGAAA334900.042.8160960
TTCTGCT332050.041.91750355
ATCTCGT317900.041.71904440
CTTCTGC338750.041.3988454
AATCTCG326000.040.6154639
AAAAGGG211650.040.1841670
Produced by FastQC (version 0.11.2)