FastQCFastQC Report
Sat 1 Sep 2018
HH2GFBGX7_n01_21_biotin_tga1_30_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2GFBGX7_n01_21_biotin_tga1_30_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18556729
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATTTTATCTCGTATGC1307990.7048602153967976TruSeq Adapter, Index 9 (97% over 36bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG425130.22909748803250832No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG262610.14151739781294428No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATTTTATCTCGTATG225120.12131448381878078TruSeq Adapter, Index 9 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC223800.047.26966544
TATGCCG228250.046.44114346
GTATGCC233100.045.955645
CTCGTAT224450.045.02350242
TCGTATG234600.043.94231443
GCCGTCT222700.043.74834449
ATGCCGT236800.043.64252547
TCTCGTA234200.042.87931441
TGCCGTC247650.041.2640348
CCGTCTT247150.040.1427550
TATCTCG263750.039.7165939
ATCTCGT265000.038.9494140
CTGCTTG257600.035.20731757
TGCTTGA265800.035.09557358
TTATCTC309100.034.2136638
GCTTGAA280900.034.13173759
AGTCACC359900.034.08300428
AAAAGGG211100.033.77009270
CATTTTA372100.032.90405334
GTCACCA378000.032.5456529
Produced by FastQC (version 0.11.2)