FastQCFastQC Report
Sat 1 Sep 2018
HH2GFBGX7_n01_20_biotin_tga1_10_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2GFBGX7_n01_20_biotin_tga1_10_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21586768
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG328630.15223677764082147No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGCA321800.036.08910834
AGTCACC333150.035.46532428
CCATGGC333650.035.2148533
ACCATGG336250.035.0130732
TGGCATC194050.034.760536
GTCACCA350750.033.708529
CAGTCAC368600.032.13698227
CATCGGG90800.031.26910239
ATGGCAT361850.031.187235
CTCCAGT397200.030.70858624
CCAGTCA385350.030.69312526
TCCAGTC401750.030.29191625
ACTCCAG409650.029.73253323
ATCGGGG66600.029.54409640
TCACCAT418350.028.4733130
GCATCGG111050.027.32981338
GGGTGTA40750.026.29352259
GAACTCC467550.026.27486821
CGGGGTG59050.026.15375351
CTGAACT483450.025.5264919
Produced by FastQC (version 0.11.2)