FastQCFastQC Report
Sat 1 Sep 2018
HH2GFBGX7_n01_1_pn_col_h3k27ac.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2GFBGX7_n01_1_pn_col_h3k27ac.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19137272
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC421340.2201672213260072TruSeq Adapter, Index 5 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG91100.053.2409646
GTATGCC97200.050.5123345
CGTATGC96200.050.4541644
ATGCCGT98900.047.8754747
TGCCGTC103100.045.65377448
GCCGTCT100450.044.7661849
CACAGTG127700.042.42623533
TCGTATG109100.042.3685143
CTCGTAT85150.040.13706242
CACACAG137950.039.3191631
GTCACAC142450.038.2171729
ACACAGT147450.036.64549632
CAGTCAC151600.035.90574327
CCAGTCA152700.035.55396726
TCCAGTC157350.034.61280425
CAGTGAT153450.033.53111635
ACAGTGA161650.032.65172634
TCTCGTA104300.032.19619441
CTGCTTG129300.032.0650957
CCGTCTT143000.031.9356250
Produced by FastQC (version 0.11.2)