FastQCFastQC Report
Sat 1 Sep 2018
HH2GFBGX7_n01_19_biotin_tga1_5_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2GFBGX7_n01_19_biotin_tga1_5_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16441159
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1870181.1374988831383481No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGCGATCTCGTATGC1284870.7814960003732098TruSeq Adapter, Index 7 (97% over 36bp)
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1242220.7555550067972702No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGCGATGTCGTATGC192390.11701729786811259TruSeq Adapter, Index 7 (97% over 36bp)
ATATACATATATATGTATATACATATATATGTATATACATATATATGTAT165460.10063767402285932No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG203300.047.079411
CTGCTTG262800.042.6762157
GCCGTCT281550.041.966349
TGCTTGA274550.041.3851858
TATGCCG303500.041.0641946
CCGTCTT295000.040.39668750
ATGCCGT307300.040.31868447
GCTTGAA290850.039.99295459
CTCGTAT253400.039.97606742
CGTATGC313050.039.84454344
GTATGCC315300.039.76072745
TGCCGTC308500.039.6395648
TCTGCTT288800.039.22298456
TCTCGTA251600.038.91068641
TCGTATG317700.038.1352343
CTTGAAA317250.037.07330760
TTCTGCT303850.036.3934355
ATCTCGT266650.036.1726340
CTTCTGC316800.036.0560554
TGAAAAA362800.035.3804762
Produced by FastQC (version 0.11.2)