FastQCFastQC Report
Sat 1 Sep 2018
HH2GFBGX7_n01_18_biotin_nlp7_180_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2GFBGX7_n01_18_biotin_nlp7_180_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29887253
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTCAATCTCGTATGC994750.3328342019254831TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC175450.047.62391744
TATGCCG182350.046.03403546
GTATGCC189500.044.42619345
GCCGTCT190350.042.72149
CTCGTAT181900.042.21654542
TCGTATG198350.041.8057243
ATGCCGT203450.041.0027747
TCTCGTA193100.039.24054741
TGCCGTC227800.036.48166348
CCGTCTT226600.036.14896450
ATCTCGT223950.033.9573640
CCACTCA263450.033.0656733
AGTCACC266550.032.66951828
CACTCAA288150.029.97814434
AATCTCG257600.029.73632439
GTCACCA296400.029.48826829
ACCACTC299450.029.1352532
CAGTCAC302650.029.00167527
CTGCTTG283850.028.33705157
CCAGTCA315550.027.8033926
Produced by FastQC (version 0.11.2)