FastQCFastQC Report
Sat 1 Sep 2018
HH2GFBGX7_n01_17_biotin_nlp7_30_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2GFBGX7_n01_17_biotin_nlp7_30_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15670984
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGATATCTCGTATGC1854751.1835568206820963TruSeq Adapter, Index 7 (97% over 35bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG261550.16690081490734723No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG241290.15397246273750265No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC282600.051.42319545
CGTATGC283300.051.27075244
TATGCCG283950.051.10471346
TCGTATG290150.049.97393843
CTCGTAT284300.049.9038542
GCCGTCT290300.049.18118749
TCTCGTA300050.047.29495241
CCGTCTT304850.047.01719350
ATGCCGT307950.046.99890547
TGCTTGA308000.045.5530158
GCTTGAA317100.044.720759
CTGCTTG313250.044.44289857
TATCTCG325400.043.6796639
TCTGCTT327700.042.7615356
TGCCGTC340500.042.35219648
ATCTCGT337750.042.1064540
ACGATAT357750.041.32733535
CTTGAAA346400.041.29168760
CTTCTGC345900.040.78671654
TTCTGCT342600.040.68781355
Produced by FastQC (version 0.11.2)