FastQCFastQC Report
Sat 1 Sep 2018
HH2GFBGX7_n01_16_biotin_nlp7_10_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2GFBGX7_n01_16_biotin_nlp7_10_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7481749
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTATGC507470.6782772317007695TruSeq Adapter, Index 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC75900.055.4599844
GTATGCC78750.053.4539345
TATGCCG79200.053.149546
TCGTATG80450.052.32018743
CTCGTAT73350.051.98707242
GCCGTCT82100.050.68036349
ATGCCGT84700.049.82660747
TCTCGTA79800.046.818541
TGCCGTC92350.045.51071548
CTAATCT84300.044.51357337
CCGTCTT95750.043.8203450
ACTAATC86350.043.4961736
AACTAAT97300.042.9895535
CCAACTA99650.042.952133
ATCTCGT87200.042.88323240
AGTCACC103850.041.33596828
TAATCTC90050.041.3227638
CAACTAA103550.041.06813434
CAGTCAC110400.040.36943427
CTGCTTG105100.039.5479857
Produced by FastQC (version 0.11.2)