FastQCFastQC Report
Sat 1 Sep 2018
HH2GFBGX7_n01_15_biotin_nlp7_5_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2GFBGX7_n01_15_biotin_nlp7_5_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6946346
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGATCTCGTATGC477130.6868791160129369TruSeq Adapter, Index 7 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC79550.050.3991244
TATGCCG80200.050.03575546
GTATGCC80350.049.85446545
GCCGTCT81900.048.7013849
CTCGTAT77650.048.15906542
TCGTATG84450.047.10099843
ATGCCGT85150.047.0889747
TCTCGTA83300.044.17703641
CCGTCTT92050.043.4079850
TGCCGTC93800.043.12018648
ATCTCGT88650.040.8348240
CTGCTTG96350.040.3390657
GATCTCG94850.037.97857739
TGCTTGA104400.037.5967558
AGATCTC97400.036.65762738
GCTTGAA108500.036.27311759
AGTCACC111300.036.1202728
GTCACCA116450.034.6445329
CAGTCAC119100.034.19340527
CTTCTGC115300.033.9220154
Produced by FastQC (version 0.11.2)