FastQCFastQC Report
Sat 1 Sep 2018
HH2GFBGX7_n01_14_ev_nlp7_gr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2GFBGX7_n01_14_ev_nlp7_gr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16004390
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT33600.034.0830449
TATGCCG36050.032.05845348
GTATGCC36800.031.59551847
CGTATGC37650.031.06678646
TGCCGTC36800.030.83398850
GCCGTCT37300.030.04397451
TCTCGTA59800.018.91430543
CCGTCTT60500.018.52251452
TCGTATG66150.017.62889745
ATCTCGT67350.017.10520742
CTCGTAT68500.016.56363744
TATCTCG70900.016.24833341
GTCACAT79300.015.44282629
ACATTCC79550.015.17741232
TTATCTC81400.014.36615340
CTGCTTG80550.013.60661759
CACATTC91700.013.31835231
GCTTGAA91550.012.66005561
TGCTTGA89850.012.43208660
AGTCACA120800.010.51346528
Produced by FastQC (version 0.11.2)