FastQCFastQC Report
Sat 1 Sep 2018
HH2GFBGX7_n01_13_ev_nlp7_h3k27ac.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2GFBGX7_n01_13_ev_nlp7_h3k27ac.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14847715
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCTCGTATGC698810.47065154469896553TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCGCGTATGC160410.10803682586849221TruSeq Adapter, Index 1 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG120200.058.00398346
CGTATGC118200.057.2944344
GTATGCC125750.056.94733445
ATGCCGT115350.055.13220247
TGCCGTC117000.051.54135548
GTCACAT245250.050.79200729
CATGAGC247000.050.27501733
GCCGTCT108250.049.91728649
CTCGTAT127300.049.1255542
CAGTCAC252900.049.06762727
CTCCAGT252800.049.0242524
ACTCCAG253350.048.97288523
TGAGCAT246500.048.0395535
TCGTATG125000.047.92985543
ACGTCTG261200.047.90508715
AGTCACA261950.047.5767528
CTGAACT263750.047.4660619
ATGAGCA263450.046.64787734
TCCAGTC267850.046.428125
CATCTCG157050.046.41257539
Produced by FastQC (version 0.11.2)