FastQCFastQC Report
Sat 1 Sep 2018
HH2GFBGX7_n01_12_ev_col_h3k27ac.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2GFBGX7_n01_12_ev_col_h3k27ac.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16035724
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT1286990.8025767966572636TruSeq Adapter, Index 25 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG162250.059.33434348
CGTATGC165950.058.5368146
GTATGCC167450.057.51232547
ATGCCGT168500.057.05002249
GCCGTCT175250.054.4513151
TGCCGTC177900.053.8585850
CACACTG182400.053.8358231
TCGTATG189100.051.37061345
CTCGTAT183000.051.3444
TCTCGTA186150.050.41212543
GTCACAC194750.050.3610229
GCACACG201100.049.5070911
ACGTCTG200950.049.43789715
ACTGATA203200.048.5388534
ACTCCAG205700.048.12562623
ACACGTC208700.047.70320513
CTCCAGT208000.047.59391424
CACACGT209950.047.46976512
CTGCTTG198750.047.42612559
CAGTCAC207000.047.40772227
Produced by FastQC (version 0.11.2)