FastQCFastQC Report
Sat 1 Sep 2018
HH2GFBGX7_n01_11_ev_col_gr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH2GFBGX7_n01_11_ev_col_gr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19416716
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGC1671530.8608716324634916TruSeq Adapter, Index 22 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG223500.060.88861546
CGTATGC225450.060.749244
ATGCCGT222350.060.70235447
GTATGCC227450.060.3084945
TGCCGTC225450.059.2778548
GCCGTCT220850.058.6413349
CGGTAGC255200.056.77766833
CACGGTA261800.055.67333631
ACGGTAG263450.055.19480532
GGTAGCA262000.054.87911634
GTAGCAT256250.054.14521435
CCGTCTT243300.053.66146550
TAGCATC243700.053.4589136
TCTCGTA235900.052.96086541
CATCTCG239600.052.4703639
TCGTATG263250.051.30512243
ATCTCGT245000.051.06086740
AGTCACG287400.050.9568428
CTCGTAT254650.050.47892442
GTCACGG301900.048.3049429
Produced by FastQC (version 0.11.2)