FastQCFastQC Report
Fri 31 May 2019
HGYTWBGXB_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYTWBGXB_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23218959
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4243841.8277477470027832No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTTTTTTTTTTT272300.11727485284762336No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG302900.031.3565983
ATCGGAA307750.030.5979822
CGGAAGA310550.030.5461354
AAAAGGG324800.030.00319580-81
GATCGGA309550.029.922251
AAGGGGG306500.029.84279882-83
ATTACGA36100.027.03307354-55
GTAGGGA195900.024.66012218-19
TATTACG33000.024.60781154-55
GAGCGTC374750.024.3871699
TCGTGTA205050.023.42328614-15
TAGGGAA196650.023.11674718-19
AAAGGGG306350.022.66947480-81
GTGTAGG216850.022.4002516-17
TGTAGGG204500.022.24305316-17
AGAGCGT413950.022.2136468
AAGAGCG431300.022.1115687
AGGGAAA241600.020.87845620-21
AGCGTCG232900.020.66908810-11
TTACGAT32450.020.34303356-57
Produced by FastQC (version 0.11.5)