FastQCFastQC Report
Fri 31 May 2019
HGYTWBGXB_n02_HFHm30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYTWBGXB_n02_HFHm30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21304561
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTTTTTTTTTTT769390.36113863130059337No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGGG692400.031.64105882-83
AAAAGGG717700.030.72051680-81
AAAAAAG957250.024.31236878-79
AAAGGGG706750.022.83530280-81
AGGGGGG773650.021.26924782-83
AAAATTT528100.020.71914928-29
AAATTTT484900.020.06705528-29
TTTGGGG656350.019.59216352-53
AAAAAGG855700.019.36502678-79
TTTTTGG634850.018.41548750-51
GGGAAAA926450.018.30182622-23
AAAAATT607250.018.26910826-27
GGGGGAA937900.017.69136220-21
TGGGGGG846900.017.64844154-55
GGGGAAA940200.017.5112622-23
GGGTTAA84600.017.06658266-67
TTTTGGG593050.016.72601952-53
GGGGGGA1019400.016.31656520-21
TTGGGGG687700.016.00870354-55
GGAAAAA1005400.015.43546924-25
Produced by FastQC (version 0.11.5)