FastQCFastQC Report
Fri 31 May 2019
HGYTWBGXB_n02_HFHm29.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYTWBGXB_n02_HFHm29.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19113552
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGGG583150.029.5206382-83
AAAAGGG607450.028.39415280-81
AAAGGGG596450.022.97257480-81
AAAAAAG810700.022.50269378-79
AGGGGGG656550.021.53554282-83
AAATTTT408000.020.47776228-29
AAAAAGG713500.019.63304578-79
AAAATTT414400.018.32186528-29
GGGAAAA760000.017.74387722-23
GGGGAAA768300.017.45326822-23
GGGGGAA773700.017.39316620-21
GGGGTAA108050.017.31926766-67
GGGTTAA73800.016.6365166-67
GGGGGGA833300.016.44272620-21
AAAAATT486500.016.1243726-27
TTTGGGG539100.016.01275352-53
AATTTTT485900.015.61082130-31
TGGGGGG762800.015.60053654-55
GGGGTTA76500.015.45951964-65
GGGTAAA125600.015.27741266-67
Produced by FastQC (version 0.11.5)