FastQCFastQC Report
Fri 31 May 2019
HGYTWBGXB_n02_HFHm28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYTWBGXB_n02_HFHm28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19511245
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG209070.10715359271025503No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGGG836000.032.9423782-83
AAAAGGG852900.032.3973680-81
CGGGTTT115150.027.5518740-41
AAAAAAG1101450.026.47921278-79
AAAGGGG843500.025.64582380-81
CCGGTTT70750.024.9049740-41
AAATTTT498700.024.51605628-29
AAAATTT527950.023.73808928-29
AGGGGGG943300.023.57772882-83
GGTTTTT466050.023.33917442-43
CGGTTTT92600.023.10587340-41
TTTTTCG104700.022.22790534-35
AAAAAGG1003250.022.08578-79
GGGGTAA128150.021.27388866-67
AAAAATT595000.020.97573526-27
GGGTTAA92300.020.71179666-67
TTTTCCG105050.019.35023736-37
GGGGTTA95250.019.0731764-65
GGGAAAA1113600.019.05796222-23
GGGTAAA146750.019.03061566-67
Produced by FastQC (version 0.11.5)