FastQCFastQC Report
Fri 31 May 2019
HGYTWBGXB_n02_HFHm27.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYTWBGXB_n02_HFHm27.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20509473
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTTTTTTTTTTT971690.47377619112885055No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG216100.10536594480023938No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGTTTTTTTTT207520.10118251210062784No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGGG950500.032.83706782-83
AAAAGGG959550.032.61611680-81
AAAAAAG1201050.027.40817878-79
AAATTTT569100.025.47673828-29
AAAGGGG950450.025.32165580-81
AAAATTT590400.024.12317528-29
AGGGGGG1072100.023.02468582-83
GGGTTAA100450.022.90875866-67
AAAAAGG1110450.022.1755378-79
AAAAATT658150.021.7955526-27
GGGGTAA146650.020.8412766-67
GGGGTTA105750.020.83993564-65
GGGTAAA164050.020.26653566-67
AATTTTT651650.020.24075130-31
GGGAAAA1238800.019.76596522-23
TTTGGGG819700.019.50649652-53
GGGGAAA1257650.019.21665822-23
GGGGGTA167950.019.20209164-65
GGGGGAA1272850.019.09031320-21
TGGGGGG1155850.018.52790854-55
Produced by FastQC (version 0.11.5)