FastQCFastQC Report
Fri 31 May 2019
HGYTWBGXB_n02_HFHm26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYTWBGXB_n02_HFHm26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21063931
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTTTTTTTTTTT460470.21860591928448683No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTGTTTTTTTTTTTTTTT334250.15868358095172264No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG241960.11486934703688499No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGGG1069950.032.6858782-83
AAAAGGG1085600.032.30168580-81
AAAAAAG1326900.027.76730278-79
AAAGGGG1074100.025.72176280-81
GGGTTAA105550.023.78169666-67
AGGGGGG1213000.023.385782-83
AAAAAGG1227950.022.96830778-79
AAAAATT688550.022.52710226-27
GGGGTTA107550.022.2795764-65
GGGGTAA155800.021.55301366-67
AAAATTT535150.021.21307828-29
TTTGGGG906950.020.95485752-53
GGGTAAA175200.020.67101766-67
GGGGGTA173750.020.21479864-65
GGGAAAA1385950.019.83881822-23
TTTTTGG889000.019.73816950-51
GGGGAAA1408850.019.32754122-23
GGGGGAA1450400.018.91019820-21
GAAAAAG461250.018.74266224-25
AAAAAAT848750.018.74245526-27
Produced by FastQC (version 0.11.5)