FastQCFastQC Report
Fri 31 May 2019
HGYTWBGXB_n02_HFHm25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYTWBGXB_n02_HFHm25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21941914
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTTTTTTTTTTT1451040.661309674260869No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTGTTTTTTTTTTTTTT494690.22545435188561944No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG266550.12147983079324803No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGTTTTTTTTTTTTTTTTTTT254860.11615212784080733No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGGG1268100.034.3694382-83
AAAAGGG1278050.034.2701580-81
AAAAAAG1556250.029.53838578-79
AAATTTT730500.027.7157628-29
AAAATTT753850.026.61470228-29
AAAGGGG1263650.026.01853480-81
AAAAATT822200.024.34490626-27
GGGTTAA116000.024.19838566-67
AGGGGGG1423350.023.5119782-83
AAAAAGG1456450.022.99304478-79
TTTGGGG1078850.022.96723752-53
GGGTAAA200050.022.53121266-67
TTTTTGG1054150.021.61781350-51
GGGGTAA196250.021.38226966-67
GGGGTTA128850.020.95566664-65
GAAAAAG508800.020.39866624-25
GGGAAAA1663850.020.1808922-23
TGGGGGG1508900.019.88357454-55
AAAAAAT988200.019.84442326-27
GTAAAAA292700.019.7966268-69
Produced by FastQC (version 0.11.5)