FastQCFastQC Report
Fri 31 May 2019
HGYTWBGXB_n02_HFHm24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYTWBGXB_n02_HFHm24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21848923
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTTTTTTTTTTT538350.24639658439914863No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGGG636350.028.81791582-83
AAAAGGG669000.027.38273280-81
AAAGGGG648900.022.95295380-81
AGGGGGG708600.021.76017682-83
AAAAAAG887350.021.6345278-79
AAAAAGG785100.019.28825678-79
GGGAAAA833800.016.86527322-23
GGGGAAA845800.016.84501522-23
AAATTTT430000.016.7405528-29
GGGGGAA862100.016.5128620-21
TTTGGGG583100.016.44931252-53
GGGTTAA83650.016.29591866-67
AAAATTT434400.016.17188328-29
GGGGGGA925550.015.98642420-21
TGGGGGG813150.015.97170354-55
GGGGTAA118050.015.89256366-67
TTTTTGG586000.015.03083650-51
GGGTAAA134850.014.86361266-67
GGGGGTA136000.014.51102164-65
GGGGTTA91150.014.2256464-65
Produced by FastQC (version 0.11.5)