FastQCFastQC Report
Fri 31 May 2019
HGYTWBGXB_n02_HFHm23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYTWBGXB_n02_HFHm23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22301328
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGGG649400.028.53546782-83
AAAAGGG664550.027.8560380-81
AAAGGGG657500.022.73617780-81
AAAAAAG901650.021.74757678-79
AGGGGGG726850.021.35463382-83
AAAAAGG793150.019.28156378-79
GGTTTTT394050.017.08041642-43
GGGAAAA843800.017.0705922-23
GGGGGAA865100.016.82899720-21
GGGGAAA864300.016.79209922-23
AAATTTT421400.016.69302728-29
AAAATTT437850.016.47809428-29
GGGTTAA83900.016.07758366-67
GGGGGGA932900.016.01834720-21
TGGGGGG829150.015.65766354-55
GGGGTTT292450.015.54979740-41
GGGGTAA122150.015.04810566-67
AAAAATT547750.015.00619526-27
TTTGGGG594400.014.9779252-53
GGGGGTA134900.014.84063364-65
Produced by FastQC (version 0.11.5)