FastQCFastQC Report
Fri 31 May 2019
HGYTWBGXB_n02_HFHm22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYTWBGXB_n02_HFHm22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22890791
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTTTTTTTTTTT814710.3559116851837929No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGTTTTTTTTTTTTTTTTTTT231880.10129837802459513No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGGG675900.030.47393682-83
AAAAGGG690500.029.9941480-81
AAAAAAG955500.022.78849878-79
AAAGGGG685300.022.59698580-81
AGGGGGG742300.021.26855382-83
AAAATTT529400.019.33081228-29
TTTGGGG645350.018.87359452-53
AAATTTT497600.018.69045428-29
AAAAAGG849400.018.65587278-79
GGGAAAA901400.017.84057822-23
GGGGAAA908650.017.35843522-23
GGGGGAA913700.017.3461120-21
TTTTTGG644050.017.31170750-51
TGGGGGG823600.017.22525454-55
AAAAATT622250.016.72537826-27
GGGTTAA90700.016.67846366-67
GGGGGGA977800.016.4373120-21
GGGGTAA126950.016.0875466-67
GGGTAAA143200.015.68815966-67
TTTTGGG590800.015.5837752-53
Produced by FastQC (version 0.11.5)