FastQCFastQC Report
Fri 31 May 2019
HGYTWBGXB_n02_HFHm21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYTWBGXB_n02_HFHm21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21523481
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGGG708100.030.0811182-83
AAAAGGG732500.029.01978580-81
AAAGGGG726550.023.7949680-81
AAAAAAG978400.022.80111378-79
AGGGGGG790850.022.65689782-83
AAATTTT482050.020.97807328-29
AAAAAGG855750.020.6071478-79
AAAATTT477500.019.4023628-29
GGGTTAA86300.018.02398166-67
GGGAAAA937500.017.84214222-23
GGGGTAA122850.017.80347366-67
GGGGGAA958100.017.54085220-21
GGGGAAA955100.017.51335722-23
AAAAATT568850.016.75438526-27
GGGTAAA139800.016.71506966-67
GGGGGGA1030300.016.67817520-21
AATTTTT566900.016.4259830-31
GGGGGTA139700.016.37009264-65
TGGGGGG923200.016.16680354-55
GGGGTTA93500.016.12810564-65
Produced by FastQC (version 0.11.5)