FastQCFastQC Report
Fri 31 May 2019
HGYTWBGXB_n02_HFHm20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYTWBGXB_n02_HFHm20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21434992
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGGG756500.031.16723482-83
AAAAGGG770500.030.74233280-81
CGGGTTT109850.025.2279840-41
AAAGGGG763300.024.90550880-81
AAAAAAG1021050.024.35906878-79
AGGGGGG843150.023.13588782-83
CCGGTTT70900.022.20644240-41
GGTTTTT453650.021.65243542-43
AAATTTT484750.021.34149228-29
CGGTTTT88900.021.28963540-41
AAAAAGG920100.021.07911778-79
AAAATTT512050.020.33817328-29
GGGGTAA132000.020.18562766-67
TTTTTCG104700.020.0733534-35
GGGTTAA90050.018.22290666-67
GGGAAAA995550.018.06877122-23
GGGGAAA1020200.017.90689822-23
TTTTCCG107400.017.887836-37
GGGGGAA1036600.017.5732920-21
AAAAATT596900.017.55483626-27
Produced by FastQC (version 0.11.5)