FastQCFastQC Report
Fri 31 May 2019
HGYTWBGXB_n02_HFHm19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYTWBGXB_n02_HFHm19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23245688
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTTTTTTTTTTT1493300.6423987106770082No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGTTTTTTTTT345330.1485565839135413No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG328770.14143268205268866No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTTTGTTTTTTT303030.13035966068201552No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGGG1422900.034.68135582-83
AAAAGGG1465350.033.85606880-81
AAAAAAG1764500.029.34723778-79
AAATTTT804550.028.50666628-29
AAAGGGG1436600.027.06411780-81
AAAATTT835000.027.05184628-29
GGGTTAA130100.025.64589966-67
AAAAATT894450.025.10050626-27
AGGGGGG1596500.024.94594282-83
GGGGTAA201450.024.17782866-67
GGGGTTA133650.023.98755864-65
AAAAAGG1655600.023.96711278-79
AATTTTT899800.023.18692630-31
GGGTAAA226500.022.67812766-67
GGGGGTA227850.022.01233764-65
GAAAAAG579550.021.71993424-25
ATTTTTT1129250.020.75747130-31
TTTGGGG1192800.020.46474552-53
GGGAAAA1918100.020.235222-23
AAAAAAT1076550.020.11786726-27
Produced by FastQC (version 0.11.5)