FastQCFastQC Report
Fri 31 May 2019
HGYTWBGXB_n02_HFHm18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYTWBGXB_n02_HFHm18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22794444
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG300240.13171630771077375No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG1155600.027.87998680-81
AGGGGGG1363350.026.12843782-83
AAAAAGG1276650.025.59702978-79
GGGGTAA193700.025.17042566-67
GGGTTAA122350.024.55374566-67
AAGGGGG1172050.024.31364882-83
AAAAGGG1152300.024.1858280-81
GGGGTTA124350.022.72661664-65
GGGTAAA211700.020.8876766-67
GGGGGTA219200.020.70397664-65
GGTAAAA253900.020.42733268-69
AAAAAAG1443150.020.15052878-79
GAAAAAG508400.019.72269624-25
GAAAAAA1341700.018.792970-71
GGTTAAA151600.018.78240466-67
GTAAAAA287800.018.6235568-69
GGGGAAA1474850.018.3784522-23
GGGGGGA1805700.017.84722120-21
GGGGGAA1566150.017.71393420-21
TTTTGGG849150.017.50220948-49
Produced by FastQC (version 0.11.5)