FastQCFastQC Report
Fri 31 May 2019
HGYTWBGXB_n02_HFHm17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYTWBGXB_n02_HFHm17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22149717
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTTTTTTTTTTT1082860.48888209271477373No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTGTTTTTTTTTTTTTT407670.1840520129444543No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG235110.1061458257006173No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGGG1004600.032.64128582-83
AAAAGGG1015500.032.42347780-81
AAAAAAG1269900.027.2122578-79
AAAGGGG1010050.025.44290480-81
AAATTTT613000.024.31538828-29
AAAATTT628200.023.60229128-29
AGGGGGG1120550.023.41350282-83
AAAAAGG1167600.022.44449878-79
GGGTTAA104250.022.39233866-67
AAAAATT701450.021.1106426-27
TTTGGGG881600.021.01084552-53
GGGTAAA171300.020.21259766-67
GGGGTAA158500.020.16683666-67
GGGGTTA112900.019.8986564-65
TTTTTGG860500.019.81232850-51
GGGAAAA1314850.019.34157822-23
TGGGGGG1186450.018.98492454-55
GGGGAAA1327600.018.8767522-23
GGGGGAA1356350.018.66985120-21
GGGGGTA189200.017.84862764-65
Produced by FastQC (version 0.11.5)