FastQCFastQC Report
Fri 31 May 2019
HGYTWBGXB_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYTWBGXB_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23218959
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4160361.7917943694202656No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT736730.3172967401337846TruSeq Adapter, Index 19 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT536760.23117315466210175TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC587700.068.425088
CGGAAGA666250.061.169464
TCGGAAG663250.061.1423763
ATCGGAA676200.060.365482
GATCGGA675250.059.6015661
GAGCACA759250.053.152339
AAGAGCA802700.051.091837
GAAGAGC844650.048.1326076
GGAAGAG904600.045.284015
TATCTCG145550.039.29003540-41
TCTCGTA268100.038.63252642-43
CGTCTGA514100.038.47753516-17
AATGCGC104200.038.13644834-35
GCTATCT148450.038.01392738-39
ATGCCGT277000.037.95633348-49
TGCGCAT109550.037.8980436-37
ACGTCTG522000.037.7453914-15
CACGTCT524250.037.73287214-15
ACACGTC527450.037.5528712-13
TAATGCG98950.037.51934434-35
Produced by FastQC (version 0.11.5)