FastQCFastQC Report
Fri 31 May 2019
HGYTWBGXB_n01_HFHm30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYTWBGXB_n01_HFHm30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21304561
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT5365262.5183621478987526TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA897700.063.0454372
GATCGGA904850.062.4567831
AGAGCAC907050.062.3625228
TCGGAAG910350.062.18433
GAGCACA911900.062.171489
CGGAAGA931800.060.7717824
AAGAGCA991100.057.174667
GAAGAGC1061350.053.4440166
GGAAGAG1205700.047.2306985
TCGTATG597700.045.59562344-45
CGTATGC605600.045.23983846-47
TATGCCG607500.045.17632348-49
CTCGTAT606400.044.85138344-45
TAATGCG627300.044.81755434-35
AATGCGC638900.044.01870334-35
TCTCGTA618800.044.01371442-43
ATCTCGT633850.042.9611742-43
TGCGCAT651800.042.95277436-37
ATGCGCA655650.042.89253636-37
ATGCCGT643450.042.54894648-49
Produced by FastQC (version 0.11.5)