FastQCFastQC Report
Fri 31 May 2019
HGYTWBGXB_n01_HFHm29.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYTWBGXB_n01_HFHm29.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19113552
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT4503542.3562025519903362TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA851400.055.136169
AGAGCAC852400.054.993478
ATCGGAA850850.054.9271772
TCGGAAG858650.054.4771543
GATCGGA864250.054.056151
CGGAAGA871950.053.656394
AAGAGCA919300.051.043117
GAAGAGC992300.047.3933566
TCGTATG494850.045.5936444-45
CGTATGC500450.045.23525646-47
TATGCCG502400.045.18713448-49
CTCGTAT503800.044.67996244-45
TAATGCG527700.044.18552434-35
TCTCGTA514000.043.77939642-43
AATGCGC535400.043.6077334-35
ATCTCGT528200.042.656442-43
ATGCGCA549700.042.44636-37
TGCGCAT549150.042.3068636-37
ATGCCGT537550.042.1528548-49
GGAAGAG1126300.041.9150545
Produced by FastQC (version 0.11.5)