FastQCFastQC Report
Fri 31 May 2019
HGYTWBGXB_n01_HFHm28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYTWBGXB_n01_HFHm28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19511245
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT7160863.6701194618795467TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA1498300.049.936839
AGAGCAC1515050.049.337718
AAGAGCA1611450.046.477737
TCGTATG783450.045.9653644-45
CGGAAGA1634050.045.7436264
CGTATGC790150.045.68657746-47
TATGCCG790750.045.6575148-49
TCGGAAG1644150.045.4541933
CTCGTAT791850.045.279844-45
ATCGGAA1666350.044.786722
TCTCGTA804350.044.6910642-43
GAAGAGC1703750.043.9821246
ATCTCGT817300.043.8870442-43
GATCGGA1701750.043.836381
ATGCCGT823550.043.80447448-49
TAATGCG856200.043.45800434-35
AATGCGC858650.043.4280534-35
GTATGCC837500.043.40984746-47
GCCGTCT826850.043.14937650-51
TGCGCAT859650.043.1164436-37
Produced by FastQC (version 0.11.5)