FastQCFastQC Report
Fri 31 May 2019
HGYTWBGXB_n01_HFHm27.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYTWBGXB_n01_HFHm27.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20509473
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT8032553.9165072647161634TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA1695750.049.003869
AGAGCAC1722650.048.2469148
TCGTATG876050.045.8749144-45
TATGCCG883800.045.7035648-49
CGTATGC884050.045.67199746-47
AAGAGCA1826550.045.538887
CTCGTAT884300.045.2669844-45
TCTCGTA895600.044.76423342-43
CGGAAGA1866050.044.548714
TCGGAAG1881550.044.1827963
ATCTCGT905750.044.16034342-43
ATGCCGT915300.044.09175548-49
TAATGCG947050.043.67875734-35
GTATGCC931000.043.6442846-47
AATGCGC950100.043.60103634-35
GCCGTCT919750.043.5499150-51
ATCGGAA1909200.043.5182572
TGCGCAT947550.043.50057636-37
GAAGAGC1922250.043.3507776
CCGTCTT929600.043.2234952-53
Produced by FastQC (version 0.11.5)