FastQCFastQC Report
Fri 31 May 2019
HGYTWBGXB_n01_HFHm26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYTWBGXB_n01_HFHm26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21063931
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT9117104.328299404322963TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA1819050.052.3672149
AGAGCAC1845000.051.568888
AAGAGCA1952050.048.8139657
CGGAAGA1977500.048.223144
TCGGAAG1990200.047.894513
ATCGGAA2013050.047.322882
GAAGAGC2050200.046.5257346
GATCGGA2058300.046.2653771
TCGTATG996250.045.91892244-45
CGTATGC1005600.045.8437646-47
TATGCCG1006200.045.84195748-49
CTCGTAT1004700.045.46180344-45
TCTCGTA1018700.044.899642-43
ATCTCGT1031700.044.3131242-43
ATGCCGT1038300.044.31034548-49
GTATGCC1054600.043.89613346-47
GCCGTCT1043250.043.68039750-51
CCGTCTT1051950.043.59893452-53
AATGCGC1088850.043.5785734-35
TAATGCG1089850.043.55602634-35
Produced by FastQC (version 0.11.5)