FastQCFastQC Report
Fri 31 May 2019
HGYTWBGXB_n01_HFHm25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYTWBGXB_n01_HFHm25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21941914
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT10987135.007370824623595TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA1893550.060.648819
AGAGCAC1913200.059.9936268
AAGAGCA2010000.057.1516467
CGGAAGA2015250.056.9642644
TCGGAAG2018500.056.8899273
ATCGGAA2030750.056.5331042
GATCGGA2061050.055.6388551
GAAGAGC2104500.054.6191756
GGAAGAG2276300.050.5970425
TCGTATG1195250.046.17573544-45
TATGCCG1209850.046.0246448-49
CGTATGC1206400.046.02057646-47
CTCGTAT1200550.045.8769344-45
TCTCGTA1219550.045.19088742-43
ATGCCGT1241900.044.7508148-49
ATCTCGT1233800.044.6862742-43
TAATGCG1279900.044.5757234-35
AATGCGC1283550.044.41565734-35
GTATGCC1257650.044.37748746-47
TGCGCAT1279450.044.34253336-37
Produced by FastQC (version 0.11.5)