FastQCFastQC Report
Fri 31 May 2019
HGYTWBGXB_n01_HFHm24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYTWBGXB_n01_HFHm24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21848923
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT5003682.290126611732761TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA894200.055.6045579
AGAGCAC894950.055.467738
TCGGAAG930850.053.379053
ATCGGAA932650.053.184972
CGGAAGA943350.052.7315644
GATCGGA951600.052.045431
AAGAGCA988550.050.3696947
GAAGAGC1071500.046.581156
TCGTATG546050.045.45894244-45
CGTATGC549450.045.0178246-47
TATGCCG551150.044.8613748-49
CTCGTAT551400.044.63023844-45
TATCTCG552100.044.40092540-41
TCTCGTA565000.043.52622642-43
ATCTCGT576600.042.50229342-43
GCTATCT576700.042.4798138-39
ATGCCGT588950.042.1111148-49
AGCTATC585300.041.7339138-39
GTATGCC598050.041.550146-47
GCCGTCT606050.040.9576850-51
Produced by FastQC (version 0.11.5)