FastQCFastQC Report
Fri 31 May 2019
HGYTWBGXB_n01_HFHm23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYTWBGXB_n01_HFHm23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22301328
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT4979462.232808736771191TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA911300.055.3942159
AGAGCAC912250.055.300088
TCGGAAG948650.053.1983453
ATCGGAA951750.052.990492
CGGAAGA962850.052.482144
GATCGGA972700.051.747191
AAGAGCA1000550.050.638257
GAAGAGC1080900.046.9706656
TCGTATG554500.045.4511444-45
CGTATGC558000.044.88504846-47
TATGCCG562200.044.59166748-49
CTCGTAT559600.044.51490844-45
TATCTCG560950.044.43671440-41
TCTCGTA575250.043.42333642-43
GCTATCT585850.042.52129738-39
ATGCCGT597300.042.24958848-49
ATCTCGT588550.042.20802342-43
AGCTATC597200.041.5421638-39
GTATGCC610250.041.38832546-47
GGAAGAG1239500.041.075515
Produced by FastQC (version 0.11.5)