FastQCFastQC Report
Fri 31 May 2019
HGYTWBGXB_n01_HFHm22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYTWBGXB_n01_HFHm22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22890791
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT5259532.297661972449969TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC930900.057.391798
GAGCACA932050.057.3821269
TCGGAAG952800.056.0981863
ATCGGAA952200.056.0645522
CGGAAGA970150.055.1330154
GATCGGA968300.055.0105131
AAGAGCA1027000.052.1972967
GAAGAGC1105450.048.5617876
TCGTATG589000.045.17279444-45
CGTATGC592950.044.7677346-47
TATGCCG592700.044.7101948-49
CTCGTAT594100.044.42922644-45
TATCTCG594550.044.19899440-41
TCTCGTA607050.043.41039342-43
GGAAGAG1256050.042.9472275
ATCTCGT622400.042.2787342-43
GCTATCT621500.042.26486638-39
ATGCCGT631150.042.14822448-49
AGCTATC629000.041.70427738-39
GTATGCC641400.041.50086246-47
Produced by FastQC (version 0.11.5)