FastQCFastQC Report
Fri 31 May 2019
HGYTWBGXB_n01_HFHm21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYTWBGXB_n01_HFHm21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21523481
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT5773462.682400676730683TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC965350.059.821118
ATCGGAA972550.059.452192
GAGCACA971950.059.4100079
TCGGAAG979100.059.0785873
GATCGGA985350.058.5755651
CGGAAGA1000750.057.8466074
AAGAGCA1054550.054.8736957
GAAGAGC1121750.051.6499256
GGAAGAG1275400.045.587695
TCGTATG633150.045.58241744-45
CGTATGC635950.045.1239946-47
TATGCCG636650.045.0628748-49
CTCGTAT638450.044.7167244-45
TATCTCG637550.044.66705340-41
TCTCGTA650200.043.95915242-43
ATCTCGT659600.043.08424842-43
GCTATCT662500.042.9465938-39
ATGCCGT674300.042.77921348-49
AGCTATC670050.042.3386238-39
GTATGCC687050.042.05130446-47
Produced by FastQC (version 0.11.5)