FastQCFastQC Report
Fri 31 May 2019
HGYTWBGXB_n01_HFHm20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYTWBGXB_n01_HFHm20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21434992
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT6237232.909835469031199TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA1352350.047.222889
AGAGCAC1358100.046.8970388
TCGTATG699900.045.44536244-45
TATGCCG700450.045.185848-49
CGTATGC703050.045.0694746-47
CTCGTAT706900.044.58547644-45
TATCTCG707650.044.4239140-41
TCTCGTA716700.043.98555842-43
AAGAGCA1475800.043.230877
ATGCCGT737300.043.1110348-49
ATCTCGT732650.042.9469442-43
CGGAAGA1485900.042.900954
TCGGAAG1487350.042.7996523
GCTATCT736950.042.60770838-39
GTATGCC746300.042.59759546-47
GCCGTCT751400.042.3491250-51
ATCGGAA1502600.042.343742
AGCTATC744850.042.0633438-39
CCGTCTT756500.041.937952-53
CTATCTC759350.041.30237240-41
Produced by FastQC (version 0.11.5)