FastQCFastQC Report
Fri 31 May 2019
HGYTWBGXB_n01_HFHm19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYTWBGXB_n01_HFHm19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23245688
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT13041765.610399657777391TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA2354950.055.976649
AGAGCAC2388500.055.1824078
AAGAGCA2516550.052.4330647
CGGAAGA2552300.051.710664
TCGGAAG2571950.051.2833373
ATCGGAA2605100.050.5783122
GAAGAGC2620950.050.3861856
GATCGGA2654700.049.5341451
GGAAGAG2809050.047.1457985
TCGTATG1433150.045.97458344-45
TATGCCG1431250.045.89734648-49
CGTATGC1434300.045.81834846-47
CTCGTAT1435750.045.5439544-45
TATCTCG1441150.045.2362340-41
TCTCGTA1445150.045.16694342-43
ATGCCGT1471050.044.78149448-49
ATCTCGT1461700.044.56292742-43
GTATGCC1482200.044.43539446-47
GCCGTCT1484650.044.3450250-51
CCGTCTT1486900.044.22825652-53
Produced by FastQC (version 0.11.5)