FastQCFastQC Report
Fri 31 May 2019
HGYTWBGXB_n01_HFHm18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGYTWBGXB_n01_HFHm18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22794444
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT10415724.569411739106249TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA1948850.055.1035359
AGAGCAC1971900.054.418478
AAGAGCA2082700.051.5736857
CGGAAGA2096650.051.1572574
TCGGAAG2101200.051.0023883
ATCGGAA2121200.050.4863432
GATCGGA2159150.049.5554771
GAAGAGC2185450.049.198916
TCGTATG1167750.045.91048444-45
CGTATGC1169100.045.6502646-47
TATGCCG1172300.045.48478348-49
GGAAGAG2377400.045.396445
CTCGTAT1171900.045.25747344-45
TATCTCG1174400.045.08955840-41
TCTCGTA1181200.044.9450342-43
ATGCCGT1209550.044.272548-49
ATCTCGT1198050.044.12061342-43
GTATGCC1217150.044.0237246-47
GCTATCT1206650.043.8334338-39
GCCGTCT1221300.043.80500850-51
Produced by FastQC (version 0.11.5)